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Pretest TriCEPS Kit

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Pretest

Flow TriCEPS / TAMRA-TriCEPS / Services/Kit


Cell selection and optimization of binding conditions for target identification




Target identification

LRC-TriCEPS / LRC-HATRIC Services


Identification of targets and off targets on the cell surface of living cells




Confirmation

Flow TriCEPS combined with siRNA knock down


Knock down the identified targets and test if ligand binding is reduced



Flow (Pretest) TriCEPS Service and Kit

LRC-TriCEPS for identification of targets – receptors of your ligand


Dualsystems-Biotech-Switzerland-Flow-TriCEPS-Principle-1


If you have adherent cells that you cannot use in for flow cytometric analyses, Dualsystems provides a TriCEPS Version that contains a TAMRA fluorophore to visualize the binding of your ligand to the cell membrane by microscopy.

Dualsystems Biotech is a service provider (and a biotech company) for custom proteomics for industry and academia. Dualsystems Biotech offers Flow-TriCEPS (Prestest TriCEPS) services and kits to support the identification of the cells to use for target identification experiments.

  • Screening of different cells
  • Cell selection
  • Positive control selection
  • Negative control selection
  • Functional Assays with TriCEPS coupled ligand
  • Flow Cytometric (FACS) reagent to analyse protein/peptide binding to cells using flow cytometry
  • TAMRA-TriCEPS reagent to analyse protein/peptide binding to adherent cells using microscopy

TAMRA-TriCEPS Service and kit

LRC-TriCEPS for identification of targets – receptors of your ligand


Dualsystems-Biotech-Switzerland-TAMRA-TriCEPS

If you have adherent cells that you cannot use in for flow cytometric analyses, Dualsystems provides a TriCEPS Version that contains a TAMRA fluorophore to visualize the binding of your ligand to the cell membrane by microscopy.

Link to Dualsystems TAMRA-TriCEPS


How does it work?



For a successful identification of the receptors or targets of a ligand (peptide, protein, antibody, ADC’s, primary amine containing molecules) it is essential that the cells used in the main LRC-TriCEPS experiment express the unknown targets. TriCEPS V2.0 can be used as reagent in Flow Cytometry (FACS) to screen different cell lines or primary cells to identify the cells that express the unknown target of the ligand (peptide, protein, antibody, ADC’s, primary amine containing molecules). For further details on the pretest experiments see this link.

Used for:

  • Screening of different cells
  • Cell selection
  • Positive control selection
  • Negative control selection
  • Functional Assays with TriCEPS coupled ligand
  • Flow Cytometric (FACS) reagent to analyse protein/peptide binding to cells

Get in contact

Dualsystems-Biotech-AG-Grabenstrasse-11a-8952-Schlieren-Switzerland-Building

The TriCEPS-based ligand-receptor capture technology is now available. Interested? Please enter your details. We are are looking forward to set up a call to discuss how we can support you best.

Call us +41 44 738 50 00 or get in touch with us using the contact form.


Pretest-TriCEPS-Kit flyer

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