Pretest
Cell selection and optimization of binding conditions for target identification
Target identification
Identification of targets and off targets on the cell surface of living cells
Confirmation
Knock down the identified targets and test if ligand binding is reduced
LRC-TriCEPS for identification of targets – receptors of your ligand
If you have adherent cells that you cannot use in for flow cytometric analyses, Dualsystems provides a TriCEPS Version that contains a TAMRA fluorophore to visualize the binding of your ligand to the cell membrane by microscopy.
Dualsystems Biotech is a service provider (and a biotech company) for custom proteomics for industry and academia. Dualsystems Biotech offers Flow-TriCEPS (Prestest TriCEPS) services and kits to support the identification of the cells to use for target identification experiments.
LRC-TriCEPS for identification of targets – receptors of your ligand
If you have adherent cells that you cannot use in for flow cytometric analyses, Dualsystems provides a TriCEPS Version that contains a TAMRA fluorophore to visualize the binding of your ligand to the cell membrane by microscopy.
Link to Dualsystems TAMRA-TriCEPS
For a successful identification of the receptors or targets of a ligand (peptide, protein, antibody, ADC’s, primary amine containing molecules) it is essential that the cells used in the main LRC-TriCEPS experiment express the unknown targets. TriCEPS V2.0 can be used as reagent in Flow Cytometry (FACS) to screen different cell lines or primary cells to identify the cells that express the unknown target of the ligand (peptide, protein, antibody, ADC’s, primary amine containing molecules). For further details on the pretest experiments see this link.
Used for:
The TriCEPS-based ligand-receptor capture technology is now available. Interested? Please enter your details. We are are looking forward to set up a call to discuss how we can support you best.
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Concerning the LRC-TriCEPS or HATRIC-LRC platforms.